Detection of Salmonella by Surface Plasmon Resonance
نویسندگان
چکیده
This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×10 cells mL and 2.50×10 cells mL, respectively.
منابع مشابه
Hydrogen sensing by localized surface plasmon resonance in colloidal solutions of Au-WO3-Pd
Nowadays, hydrogen has attracted significant attention as a next generation clean energy source. Hydrogen is highly flammable, so detection of hydrogen gas is required. Gold nanoparticle based localized surface plasmon resonance (LSPR) is an advanced and powerful sensing technique, which is well known for its high sensitivity to surrounding refractive index change in the local environment. We p...
متن کاملAntibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra
In this paper, a fast and sensitive localized surface plasmon resonance (LSPR) based biosensor was developed and the optimization of gold – antibody conjugates through investigation of different parameters were performed. Gold nanoparticles (AuNPs) with a size of ~20 nm were synthesized via chemical reduction of HAuCl4 with trisodium citrate as reducing and stabilizing agent. The impacts of pH ...
متن کاملInvestigating the Effects of Some Capping Agents upon Gold Nanocrystals Stability, Size and Surface Plasmon Resonance
متن کامل
Behenic Acid Monolayer and Bilayer Assemblies- A Study of Concanavalin A (Con A) Adsorption and its Interaction with Dextran Using Surface Plasmon Resonance Spectroscopy and Microscopy
Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...
متن کاملMicrocontact Imprinted Plasmonic Nanosensors: Powerful Tools in the Detection of Salmonella paratyphi
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and the...
متن کامل